Letters in Biotechnology 2011 Issue 5 Prokaryotic Expression and Purification of Flagellins and Their Mutants as the Neotype Adjuvants

Prokaryotic Expression and Purification of Flagellins and Their Mutants as the Neotype Adjuvants
WU Xiao-Fang;LI Shu-Long;DAI Hong-Mei;FU Gui-Ming;FANG Hong-Qing

..............page:691-695

Expression of Human Glutamine:Fructose-6-Phosphate Amidotransferase and its Biological Activity Detection
LIU Qia;ZHA NG Xiao-Lin;JIN Jing;TIAN Jin-Ying;YE Fei

..............page:612-616

CCCTC-Binding Factor Interacts with Importin 13
WANG Rong;SHEN Jing-Jing;HUANG Pei-Tang;ZHU Xu-Dong

..............page:640-642

sheng wu ji shu tong xun zheng gao jian ze

..............page:642

Construction and Identification of Eukaryotic Expression Plasmid of Porcine Endogenous Retrovirus env Gene
YE Xiao-Li;L(U) Mao-Min;YAN Qi-Po;GUO Yi;WU Jian-Min;TIAN Ke-Gong;MA Yu-Yuan;ZHANG Jin-Gang

..............page:683-686

Establish and Preliminary Application of RT-LAMP Technique in Detection for Influenza A(H1N1) HA Gene
WANG Xiang;ZHANG Qian;ZHA NG Fang;YUAN Wu-Mei;MA Fen-Lian;XUE Peng-Hao;ZHAO Zhi-Hui;ZHENG Wen-Zhi;ZHENG Li-Shu

..............page:696-699,708

Study on Optimizing the Method of Electrotransfection Applying CHO cell by Orthogonal Design
LU Jian;LI Ping;MA Ya-Ru;CHEN Yong;JIANG Lin

..............page:713-716

Establishment of Direct Competition ELISA Method for the Detection of Mouse Nerve Growth Factor
ZHA0 Qiang;DING Hong-Mei;LIU Nong-Le;LI Shao-Hua;LI Jie;XIA Wei;LI Hui;WANG Fang;LIU Chuan;SHAO Ning-Sheng

..............page:705-708

Expression and Regulation of Ypel3 Gene During Mouse Embryo Development
SHEN Yi;HAN Zheng-Bin;ZHANG Feng-Wei;GU Ning;CHEN Yan;WU Qiong

..............page:643-646

Construction and Expression of TBK1 Kinasedead Mutant and Ubiquitin-like Domain Mutant in 293 Cell Lines and Identification of theirs Biological Activity
WEI Cong-Wen;GUA N Kai;YUE Xiang;YUAN Yuan;SONG Ting;ZHENG Zi-Rui;ZHANG Yan-Hon;ZHONG Hui

..............page:671-674

Identification of CTL Epitopes in Nucleoprotein of H5N1 Influenza Virus by Using HLA Transgenic Mice
RU Zhi-Tao;KOU Zhi-Hua;LI Jun-Feng;SUN Shi-Hui;ZHAO Guang-Yu;GUO Yan;DONG Yu-Chun;ZHOU Yu-Sen

..............page:657-661

Expression of Neuron-Restrictive Silencer Factor and Insulin in the Pancreas of Adult Mice
CHEN Lin;GUO Xiao-Li;YUE Wen;L(U) Yang;XIE Xiao-Yan;YAO Hai-Lei;CHEN Hai-Xu;NAN Xue;LI Yan-Hua;PEI Xue-Tao

..............page:608-611

Isolation and Primary Analysis of High Temperature Responsive EST from Draba stenocarpa
LIU De-Tuan;LI Wan-Sha;YANG Yong-Ping;HU Xiang-Yang

..............page:617-622

Real-Time Quantitative PCR Technology and its Application in Detection of Infectious Diseases
ZOU Hong-Xia;LIA NG Guo-Lu

..............page:751-754

Establishment and Application of Loop-Mediated Isothermal Amplification for Detection of Mycobacterium bovis
xie zhi qin ; xie zhi xun ; liu jia bo ; pang yao shan ; deng xian wen ; xie li ji ; peng yi ; fan qing

..............page:709-712

Construction and Application of a Novel Dual-Luciferase Reporter System Used for Identification of microRNA Target Gene
BAO Chun-Yang;LI Jun-Feng;ZHANG Hui-Na;ZHA NG Hong-Fei;SUN Shi-Hui;YU Hong;ZHOU Yu-Sen

..............page:651-656

A LC-MS/MS Assay for the Quantification of O-Desmethylvenlafaxine in Beagle Plasma and Applications to Preclinical Pharmacokinetic Studies on Desvenlafaxine Fumarate Administered to Beagles
SHI Hong-Jiao;MENG Qing-Fang;CHEN Zhi-Hang;LIU Yun-Long;SHAN Cheng-Qi;XU Xian-Xing;CHE Jin-Jing;TAN Li;HOU Yu-Nan;CHENG Yuan-Guo

..............page:717-722

Expression and Purification of CagA Amino Terminal Fragment from Helicobacter pylori and Interaction Between CagA with Phosphatidylserine
GUA N Zhang-Yan;WANG Peng;TAO Hao-Xia;YUA N Sheng-Ling;WANG Yan-Chun;SHEN Yan-Jie;WANG Ling-Chun;LIU Chun-Jie

..............page:631-635

Expression in Escherichia coli and Purification of GST tag TRAF6
SUN Li;WANG Xiao-Hui;GUAN Wei;TANG Liu-Jun;WANG Jian;YANG Xiao-Ming;WANG Si-Ying

..............page:679-682

Prokaryotic Expression and Purification of the Tick-Borne Encephalitis Virus Recombinant E Protein Domain Ⅲ and Identification Based on Enzyme-Linked Immunosorbent Assays
WANG Dan;KANG Xiao-Ping;HAO Huai-Jie;ZHENG Yu-Ling;JIA NG Yong-Qian;YANG Yin-Hui;CHEN Fu-Sheng

..............page:636-639,666

Determination of Disulfide Bond Linking Mode for Rrythropoiesis Stimulating Protein
SANG Zhi-Hong;XUE Yan;ZHAO Yong-Qiang;LIU Bing-Yu;WANG Hong-Li;LI Ping;YANG Song-Cheng;HE Kun

..............page:700-704

Research on Optimum Fermentation Condition of Recombined Human Antithrombin
FENG Qiu-Yue;LI Wei-Jing;HE Min;SHI Xiao-Yue;QIAO Zhi-Xin;YANG Xiao-Cong;CHENG Hong;ZHANG Gong-Qing;WANG Jing;GONG Ning;WANG Xuan-Lin;YU Qun

..............page:687-690

Progress on the Molecular Genotyping Techniques of Bacillus anthracis
WU Song-Yu;LIU Xian-Kai;SONG Li;WEI Hua;WANG Heng-Liang

..............page:738-742

LRP16 Affects Ionizing Radiation-Induced NF-κB Activity
MA Xiao-Xing;WU Zhi-Qiang;DUAN Yu-Kun;MENG Yuan-Guang;HE Wen-Jun;ZHAO Ya-Li;LI Xiao-Lei;HAN Wei-Dong

..............page:627-630,670

Soluble Expression of EC1 Fragment of Human Corticotropin-Releasing Factor Receptor Ⅰ in Prokaryotic System
YU Jin-Mei;MA Xiao-Yun;SUN Hong-Liang;YAN Hai-Tao;ZHENG Jian-Quan

..............page:675-678,682

Analysis of the Results of Antinuclear Antibody Spectrum for Patients of Moyamoya
TIAN Shu-Guang;CHEN Jian-Kui;YU Nong;SONG Shi-Ping;YIN Xiu-Yun;HUANG Yuan;JIN Xin;ZUO Xiang-Hua;DU Yu;ZENG Li-Jun

..............page:723-725

Identification of Hepatocyte Nuclear Factor 3β Protein Complex by IP/MS
SUN Ting-Ting;SONG Li-Na;YU Miao;LI Chang-Yan;YANG Xiao-Ming;WANG Si-Ying

..............page:662-666

Gene Cloning and Expression of Human TBK1 in 293 Cell Lines and Identification of its Biological Activity
WEI Cong-Wen;GUAN Kai;YUE Xiang;YUAN Yuan;SONG Ting;ZHENG Zi-Rui;ZHANG Yan-Hon;ZHONG Hui

..............page:667-670

Detection of the Expression of Neuron-Restrictive Silencer Factor in the Model of Neuronal Differentiation from NTera2/CloneD1 Cells
ZHANG Jing;WANG Si-Han;ZHA NG Bo-Wen;YAO Hai-Lei;CHEN Lin;L(U) Yang;NAN Xue;YUE Wen;LI Yan-Hua;PEI Xue-Tao

..............page:603-607

Genomic DNA Extraction from Apis cerana cerana Preserved by Different Methods
YAN Hua-Chao;JIA Shao-Bo;WANG Xue-Mei

..............page:726-728,732

Trends in Detection Methods of Ricin Toxin
JIA Ming-Xian;YIN Hui-Qion;WANG Yue-Hua;ZHANG Jin-Gang

..............page:747-750

Progress on Molecular Diagnosis of Respiratory Viruses
YU Xio-Yan;L(U) Jian-Xin;TAN Wen-Jie

..............page:743-746

Investigate the Interaction Between LRP16 and Function Epi-Position of NF-κB/p65 by GST-Pulldown in vitro
LI Xiao-Lei;WU Zhi-Qiang;MA Xiao-Xing;ZHAO Ya-Li;HAN Wei-Dong

..............page:623-626

Progress of TRAIL-Based Cancer Treatment Strategies
SUI Zhen;YIN Bang-Qi;LI Zhao-Fa

..............page:733-737

A Method for Biotinylation,Purification and Detection of Enhanced Green Fluorescent Protein Based on Avi-tag Technology in Human Embryonic Kidney Cells
LI Dan-Dan;LI Ying;WU Xiao-Tao;LU Yun-Xia;WANG Xue-Jun;WANG Sheng-Qi

..............page:647-650,666

Progress and Perspective of the Induced Pluripotent Stem Cells Technology
CHEN Xiao-Ying;MIA O Xiang-Yang;WANG Jian-Min;XIE Zhi-Jing

..............page:729-732